Health / Medical Topics |
Bisulfite Sequencing
A DNA sequencing technique that can differentiate cytosine from 5-methylcytosine in a DNA sample. First, a denatured DNA sample is treated with bisulfite which converts non-methylated cytosine to uracil. Next, the sample is amplified using a PCR method that does not discriminate between non-methylated and methylated sequences. The amplified DNA is subjected to nucleotide sequencing. The resulting sequence is compared to an identical control sample of DNA that was not treated with bisulfite. Unmethylated cytosines will be displayed as cytosines in the control sample and as thymines in the bisulfite-treated sample. (NCI Thesaurus)
YOU MAY ALSO LIKE
Location on a DNA strand where the phosphate backbone is intact but the nitrogenous base has been lost from both DNA…
The necrosis (death) of bone tissue caused by treatment with a bisphosphonate (a drug or substance used to treat osteoporosis, bone pain…
A drug or substance used to treat hypercalcemia (abnormally high blood calcium) and bone pain caused by some types of cancer. Forms…
An antibody that can bind to two different antigens at the same time. Bispecific monoclonal antibodies are being studied in the imaging…
An antibody with potential antineoplastic activity. Specific for both the high-affinity immunoglobulin G (IgG) receptor CD64 and epidermal growth factor receptor (EGFR),…
A humanized bivalent antibody directed against both cytotoxic effector cells expressing Fc gamma receptor type I (Fc gammaRI, or CD64) and HER2/neu-overexpressing…