CD34/TK75 Retroviral Vector-Transduced Donor Lymphocytes

A preparation of donor T-lymphocytes that are transfected with a retroviral vector encoding a chimeric suicide gene consisting of the extracellular and transmembrane domains of human CD34 and mutant 75 of the herpes simplex virus thymidine kinase (HSV-TK75) with potential controllable immunomodulating activity. Donor T cell therapy following allogeneic hematopoietic stem cell (HSC) transplantation may result in a graft-versus-leukemia (GVL) and help control transplant-related viral infections. In the event that graft-versus-host disease (GVHD) develops due to donor lymphocyte infusion, CD34/TK75-transduced donor lymphocytes may be selectively eliminated by administration of the prodrug antiviral agent ganciclovir GCV. In CD34/T75-transduced donor lymphocytes, GCV is phosphorylated by expressed HSV-TK75 to its monophosphate form and, subsequently, converted into its active triphosphate form, which specifically kills the donor lymphocytes. The expressed CD34 moiety of the chimeric suicide gene serves as a selection marker; mutant 75 of HSV-TK confers increased GCV sensitivity. (NCI Thesaurus)

YOU MAY ALSO LIKE

CD34 wt Allele

Human CD34 wild-type allele is located within 1q32 and is approximately 40 kb in length. This allele, which encodes hematopoietic progenitor cell…

CD34 Selection

CD34 is a protein found on the surface of some bone marrow and blood cells. CD34 selection is a process where…

CD34 Measurement

The determination of the amount of CD34 expressing cells present in a sample.

CD34 Gene

This gene is involved in hematopoiesis; however, a specific physiological function has yet to be identified.

CD34 antigen

A protein found on the surface of some bone marrow and blood cells.

CD33CAR-CD3zeta-4-1BB-expressing Autologous T-Lymphocytes

Autologous T-lymphocytes transduced with a retroviral vector expressing a chimeric antigen receptor (CAR) consisting of an anti-CD33 scFv (single chain variable fragment)…