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    Cell-free System

    Definition 1

    A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166) (NLM, Medical Subject Headings)

    Definition 2

    experimental condition created by disrupting cells with homogenization, sonication, detergents, or other treatment, leaving intracellular components exposed to controlled manipulation of the medium. (NIH CRISP Thesaurus)




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