| Health / Health News |
Scientists restore the vision of blind rats for 8 months thanks to artificial retina injected into the eye
An international team of scientists, of which the University of Granada (UGR) is a member, has developed a new nanoparticle-based artificial retina prosthesis that can be injected into the eye. Thanks to this important scientific finding, blind rats had their vision restored vision for 8 months without the need for surgery.
Upper row: Confocal fluorescence microscopy image showing nanoparticles (in red) deposited on the neuronal membranes (in dark blue) without entering the cells. Bottom row: Scanning electron microscopy image showing a neuron on a layer of nanoparticles and the section of a cell whose membrane has formed with the presence of a non-internalized nanoparticle. Photo: University of Granada
The research was conducted by researchers from the Centre for Synaptic Neuroscience and Technology at the Center for Nano Science and Technology (Istituto Italiano di Tecnologia, Genoa, Italy), in collaboration with several colleagues from the Universities of Pisa, Genoa, Milan, and Granada, on the one hand, and Genoa, Negrar, and Mantova Hospitals, on the other. Among the scientists involved is Mattia Bramini of the Istituto Italiano di Tecnologia.
Hereditary retinal dystrophies and age-related macular degeneration, which are among the most common causes of blindness, have long been very difficult to treat.
Modern retinal prostheses have been developed to stimulate the inner retinal network, but the lack of sensitivity, the poor resolution, and the need for wiring or external cameras have greatly limited their application.
In the present study, the researchers successfully demonstrated how conjugated polymer nanoparticles (P3HT-NP) can mediate the light-evoked stimulation of retinal neurons and consistently recovered the visual functions of blind rats.
To achieve this result, the nanoparticles were tested and injected subretinally in a rat model of retinitis pigmentosa. “In the model we studied, the nanoparticles stimulated the light-dependent activation of the intact internal retinal neurons, recovering visual responses with no inflammation of the retina,” explains Bramini.
“Given that they achieved light sensitivity following a single injection, and with the potential for high spatial resolution, nanoparticles provide a new way forward in retinal prostheses, with potential applications not only in the case of retinitis pigmentosa but also in age-related macular degeneration.”
One of the primary advantages of the new solution identified by this scientific team, which taps into the enormous potential of multifunctional materials at the nanoscale, is the higher spatial resolution they offer compared to existing two-dimensional prostheses.
“Furthermore, the nanoparticles measure ≈300 nm in diameter (300 times smaller than the diameter of a hair), which enables them to remain extracellular to the neurons while maintaining high biocompatibility,” explains Bramini.
When microinjected into the eyes of blind rats, the nanoparticles are widely and consistently spread throughout the subretinal space, without any significant inflammatory reactions occurring.
After just a single injection, the nanoparticles recuperate the physiological behavior of the retina in light, as well as the activity of the visual cortex and visual acuity, at levels indistinguishable from those of healthy rats. This effect was found to last up to 8 months. (University of Granada)
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